Software

Open-source tools developed in the lab. Free to use. Please cite the linked DOI.

New Python Deep learning MIT licence

VesEvoL Vesicle Evolution over Long time

Tanmay Pandey, Rajni Kudawla, Jeyanth Varshan B S, Chetana Devarakonda, Aritra Chatterjee, Tripta Bhatia

Description: An image-analysis framework for time-lapse microscopy (CZI / TIFF stacks) that follows vesicles over extended time periods. It detects the boundary of a large "mother" vesicle in every frame, detects and tracks the smaller "daughter" vesicles (StarDist, Cellpose, trackpy), and classifies mother–daughter and daughter–daughter contacts. It also provides curvature, normalised-area, polar and segmentation-validation analyses, plus figure generation.

Publication:
  1. Evolution of Vesicle Shape Parameters Based on A Deep-Learning Based Image Analysis Framework Over Extended Time Periods. Pandey et al., bioRxiv (2026)
Python GUI

Shape analysis tool

Tanmay Pandey, Tripta Bhatia

Description: A Python-based GUI tool that analyses three-dimensional stacks of confocal images and returns the relevant shape parameters.

Publications:
  1. Experimentally Determined Shapes of Plasma Membrane Vesicles, Phosphatidylcholine (PC), and PC-Cholesterol Vesicles: Vesicle Deflation Analysis Using Confocal Microscopy. Harshmeet et al.
  2. Shape Analysis of Biomimetic and Plasma Membrane Vesicles. Rajni et al.
Web app

Membrane Mechanics

Avneesh Singh, Ridima Singh, Tripta Bhatia

Description: An interactive analysis for Giant Unilamellar Vesicles (GUVs). Compute angular auto-correlations and extract the bending rigidity κ and effective surface tension Σ via Helfrich / Brochard–Lennon theory.

Open GUV Flicker
Web app Teaching

Spherical Harmonics Explorer

Avneesh Singh, Ridima Singh, Tripta Bhatia

Description: Interactive software that visualises spherical harmonic functions Ylm(θ, φ). Users can explore how the quantum numbers l and m affect the shape, symmetry and nodal structure of the functions on a sphere.

Open the Explorer